Skip to main content Skip to search Skip to main navigation

Real-time PCR-system QPCR-500

Product information "Real-time PCR-system QPCR-500"

High specification, economically priced real-time thermocycler. The QPCR-500 system has a unique high thermal block uniformity (±0.1 °C). This uniformity as well as validated sample volumes of down to 5 µl enable an optimized reaction run time for 40 cycles down to only 15 minutes. The uniform temperature across the plate is achieved with a unique heating and cooling system that quickly enables a uniform temperature of ±0.1 °C in every well of the block within a fraction of a second of reaching each well temperature. This high uniformity eliminates the need for triplicates which reduces reagent running costs. QPCR-500 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescent signal. Applications include e.g. absolute and relative quantification, Allelic Discrimination and High Resolution Melt.

Features:
  • Validated volume per well: 5 to 20 µl

  • High temperature uniformity of ±0.1 °C across the whole block instantly after every temperature change

  • 40 cycles in 40 minutes before optimization, and down to only 15 minutes when optimized

  • Genotype in 4 minutes with over 99 % accuracy

  • Patented "Adaptive LED Control" minimizes optical artifacts and prevents premature detector saturation

  • Dual LED excitation (452 nm to 486 nm and 542 nm to 582 nm)

  • 4 emission filters (505 nm to 545 nm, 562 nm to 596 nm, 604 nm to 644 nm, 665 nm to 705 nm)

  • Data is always collected in all four filters for all wells

  • Distinguishes 5000 and 10000 template copies with 99 percent confidence


Additional features:
  • 48-well block

  • MIQE compliant

  • 400 analyte specific qPCR reagent kits available

  • No calibration required

  • Calibrated dyes: SYBR®, FAMTM, HEXTM, ROXTM, Cy®5.

  • Additional dyes within the filter wavelength range are supported with no additional calibration required

  • Unlimited software licence

  • 2 years warranty


Scope of supply: Real-time PCR-system QPCR-500, EcoControl and EcoStudy software, USB stick, 50 plates, 50 plate seals, power and Ethernet cable

Depth: 310 mm
Dimensions (WxDxH): 345 x 310 x 320 mm
Height: 320 mm
Manufacturer Article Number: 93947-00
Max. temperature: 100 °C
Max. voltage: 240 V
Min. temperature: 30 °C
Min. voltage: 100 V
No. of blocks: 1
Power supply: 100 ... 240 V, 50/60 Hz
Power supply frequency: 50/60 Hz
Temperature: 30 ... 100 °C
Temperature accuracy: ±0,1 °C
Type: QPCR-500
Type of auxiliary energy: Mains connection
Warranty: 2 years
Weight: 13.6 kg
Width: 345 mm