Plasmid Purification-Kits I-Blue Mini / Midi
Content:
1
Product number:
4661769
Product information "Plasmid Purification-Kits I-Blue Mini / Midi"
For rapid and efficient extraction of plasmid DNA from bacterial cells.
The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization.
The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization.
- Fast extraction time
- High yield
- Prevention of handling errors during cell lysis and neutralisation by I-Blue colour indicator
| Specifications | I-Blue Mini Plasmid Kit // I-Blue Midi Plasmid Kit |
| Method: | Spin column // Anion-Exchange |
| Sample size: | 1 - 7 ml // 50 - 100 ml high-copy plasmid / 100 - 150 ml low-copy plasmid |
| Binding capacity: | 50 µg // 500 µg |
| Fragment size: | 1 - 15 kb // 1 - 20 kb |
| Typical yield: | up to 50 µg // 200 - 500 µg |
| Operation Time: | 15 min. // 80 min. |
| Description: | Spin column |
|---|---|
| For: | 300 preps |
| Manufacturer Article Number: | IB47172 |
| Type: | I-Blue MINI |