Blotting paper
Recommended for blotting techniques with gels, like Southern, Northern and Western Blots, even Semidry-Blots of proteinsMade from ultrapure cotton linters and cellulose, entirely without additives to ensure that no contamination will occur during the transfer stepsTested specifically for chromatographic and blotting techniques to ensure high and uniform capillary actionsHigh wet strength for safe handlingFurther sizes on request.
Staining Solution Ponceau S
For staining blotted proteins on nitrocellulose and PVDF membranes. Colour detection for immunoblots (Western blots). Can also be used for staining proteins on cellulose acetate membranes.Membrane stainingReversible colouringReusable
Sub Blot Systems omniBLOT
The omniBlot is an stand-alone unit for the tank-blot method. Each unit consists of an OmniPAGE tank and lid, a electroblotting insert as well as cassettes and fibre pads. The versatile design of the tanks and the matching lids are compatible with the vertical PAGE or IEF systems with the appropriate optional inserts.Mini system transfers up to 4 Mini gelsMini Wide system transfers up to 4 Mini Wide and 8 Mini gelsMaxi system transfers up to 4 Maxi gels, 8 Mini Wide and 16 Mini gelsRun-times ranging between 2 and 20 hoursRigid cassettes eliminate the formation of shadow bandsColour coded cassettes prevent reverse polarity for correct orientationCooling with cooling packs at high transmission intensitySpecificationsDimensions (W x D x H):Mini: 15 x 13 x 19 cm
Mini Wide: 26 x 16 x 16 cm
Maxi: 27 x 18 x 30 cmGel dimension:Mini: 8 x 8.5 cm
Mini Wide: 16 x 8.5 cm
Maxi: 16 x 17.5 cmMax. buffer volume:Mini: 1200 ml
Mini Wide: 2800 ml
Maxi: 6400 mlRun time:Mini: 1-2 h
Mini Wide: 1-2 h
Maxi: 5-20 hPower supply:100 V
Blocking solution for Western blot
An all-in-one blocking solution for Western blot analysis. In one step, blocking, primary and secondary antibodies hybridization as well as enhancing the signal.Enhance antibody signalUniversal antibody diluentEffective with any ECL substratesCompatible with PVDF and NC membraneImprove protein detection
Substrate kits, ECL
ECL substrate with stable light output. The formulation provides a low background for a high signal to noise ratio.Test kit includes luminol / enhancer and peroxide solutions.LumiGO: For low picogram detection. 125 ml luminol/enhancer solution, 125 ml peroxide solution.LumiPRO: For low femtogram detection and an very high signal intensity. 50 ml luminol/enhancer solution, 50 ml peroxide solution.Long signal durationWorking solution stable for at least three days
Transfer membrane, supported nitrocellulose
Supported Nitrocellulose transfer membranes combine the binding characteristics of nitrocellulose membranes with the strength of nylon membranes. These membranes are used for DNA/RNA transfers requiring low background or for multiple hybridizations. For use with chemiluminescence and radiographic detection systems. BSA binding capacity up to 100 µg/cm2.Signal word:H phrases:P phrases:
Transfer membrane, PVDF
Hydrophobic Polyvinylidene Fluoride (PVDF) transfer membrane with high binding capacity and low background. Ideal for use in protein binding applications such as Western blots, solid phase assays and immunoblotting procedures. BSA binding capacity up to 125 µg/cm2
Transfer membrane, nitrocellulose
Pure Nitrocellulose transfer membrane for all protein or immunoblotting applications. The high sensitivity and low background ensures excellent results in all transfers. Compatible with all detection systems. BSA binding capacity up to 100µg/cm2.Signal word:H phrases:P phrases:
Blotting papers
High quality cotton papers with high absorbency for use in all blotting methods with gels.
Semi Dry Blotters
These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting; typically 15 to 30 minutes. All units can be used for all types of blotting: western, southern and northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. Each unit is compatible with their respective omniPAGE vertical gel system. Semi Dry Blotting has the added benefit of economic transfers due to very low buffer volumes - typically only a few millilitres of buffer are required per transfer. These Semi-Dry Blotters utilise a screw down lid, which secures the blot sandwich and allows complete control of pressure ensuring even transfer. The electrodes, comprising platinum coated anode and stainless steel cathode, will exhibit practically no corrosion and so provide many years of trouble free use. Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. Being translucent, it allows viewing of the blot sandwich to ensure correct positioning and transfer is occurring correctly. Electrode plates are fully separated to prevent arching or damage.
Gel blotting paper
GB 003, GB 005. High purity. Very absorbent. Easy to use. Suitable for all protein and nucleic acid blotting techniques.
Transfer membranes Amersham Protran
Pure nitrocellulose with extreme mechanical stability - gives excellent useability. For protein transfer and DNA/RNA transfer techniques. Optimum signal/background ratio.
LLG-Transfer membrane, supported nitrocellulose
Supported Nitrocellulose transfer membranes combine the binding characteristics of nitrocellulose membranes with the strength of nylon membranes. These membranes are used for DNA/RNA transfers requiring low background or for multiple hybridizations. For use with chemiluminescence and radiographic detection systems. BSA binding capacity up to 100 µg/cm2.The safety data sheets of these products are available under www.llg-labware.com.
Membrane Tweezers
Swiss made. Made of austenitic steel. Non-magnetizable. Good corrosion resistance to most chemicals, salts and acids. Especially suitable for safe handling of transfer membranes.
GEL blotting paper
Extremely pure, very absorbent, smooth paper for use in all protein and nucleic acid blotting techniques.
Storage boxes, HDPE with tightly closing LDPE lid
NaturalTemperature range (without mechanical stress) from -20 to 95°CAcid and chemical resistantSuitable for cold storage rooms and refrigeratorsStackableFood safe
Transfer System Nytran SuPerCharge Turboblotter™, refill pack
The TurboBlotter is very easy to set up, and works without power or vacuum source. Offers high resolution transfer of DNA and RNA with unique downward capillary transfer in one hour. Reusable blotting device requires less buffer and blotting paper. Scope of supply: 5 transfers
Transfer membranes Amersham Hybond P, PVDF
Hydrophobic membrane with high physical strength, suitable for stripping and reprobing. For use with standard colourimetric and chemiluminescent detection methods for proteins of < Mr 20 000.Protein binding capacity over 200 µg/cm2High protein retention even after harsh washing stepsAvailable in popular sizes for laboratory application
Transfer membranes Optitran®
Webbing reinforced nitrocellulose.High mechanical stability for multiple hybridisations.
Transfer membranes Amersham Hybond SEQ, PVDF
Hydrophobic Western blotting membrane specifically designed for protein sequencing applications and solid-phase amino acid analysis.High protein binding capacityChemical resistance needed for N-terminal sequencingHigh protein retention even after harsh washing stepsMinimizes "blow-through" and increases protein bindingAvailable in popular sizes for laboratory application
Transfer membranes Amersham Hybond LFP, PVDF
Low fluorescent, hydrophobic blotting membrane for protein transfer. Optimized for fluorescence detection in Western blotting applications.Protein binding capacity over 200 µg/cm2Higher sensitivity due to low background fluorescenceCompatible with many blocking agents, transfer buffers and total protein blot stainsAvailable in the standard precut sizes for laboratory applications