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Acylation reagents for GC - Anhydrides
For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.Reagents for silylation - alkylation (methylation) - acylation are available.Acylation with fluorinated acid anhydrides can be used for alcohols, phenols, carboxylic acids, amines, amino acids and steroids forming volatile, stable derivatives suited for FID as well as for ECD detection.by-products of acylation with anhydrides: corresponding acidsexcess reagent and the acid formed have to be removedHeptafluorobutyric acid anhydride (HFBA)m.w. 410.06, Bp 106 - 107°C (760mm Hg),C3F7 - CO - O - CO - C3F7density d20°/4° = 1.665
Silylation reagents - BSTFA, SILYL-991
For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.Reagents for silylation - alkylation (methylation) - acylation are available.N,O-bis-trimethylsilyl-trifluoroacetamidem.w. 257.4, Bp 40°C (12mm Hg), density d20°/4° = 0.961BSTFA: R = CF3 powerful trimethylsilyl donor with approximately the same donor strength as the non-fluorinated analogue BSAadvantage of BSTFA over BSA: greater volatility of its reaction products (particularly useful for GC of some lower boiling TMS amino acids.BSTFA is nonpolar (less polar than MSTFA), and can be mixed with acetonitrile for improved solubility. For silylating fatty acid amides, hindered hydroxyls and other compounds, which are difficult to silylate (like secondary alcohols and amines), we recommend BSTFA + 1% trimethylchlorosilane (TMCS), available under the designation SILYL-991.Due to their purpose, derivatisation reagents are very reactive substances. For this reason they should be stored cool and protected from moisture.The derivatisation reagents are supplied in vials with crimp caps for easy access with a syringe. Vials with pierced sealing disks have limited stability and should be used immediately.
Silylation reagents - MSTFA
For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.Reagents for silylation - alkylation (methylation) - acylation are available.N-methyl-N-trimethylsilyl-trifluoroacetamidem.w. 199.1, Bp 70 °C (75 mm Hg), density d20°/4° = 1.11MSTFA: R' = CF3, R" = CH3The most volatile trimethylsilyl amide available.Very strong TMS donor which does not cause any noticeable fouling of the FID burning chamber even after long-time measuring series.The already good solution characteristics can be improved by addition of submolar quantities of protic solvents (e.g. TFA for extremely polar compounds such as hydrochlorides) or pyridine (e.g. for carbohydrates).Due to their purpose, derivatisation reagents are very reactive substances. For this reason they should be stored cool and protected from moisture.The derivatisation reagents are supplied in vials with crimp caps for easy access with a syringe. Vials with pierced sealing disks have limited stability and should be used soon.
Alkylation reagents for GC - Trimethylsulphonium hydroxide
For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.Reagents for silylation - alkylation (methylation) - acylation are available.TMSH (0.2 M in methanol) M.G. 94.06
Alkylation kit
For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.Reagents for silylation - alkylation (methylation) - acylation are available.Which is the proper reagent for methylation?Test kit for determination of the optimum alkylation.
Derivatisation method development kit
For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.Reagents for silylation - alkylation (methylation) - acylation are available.Which type of derivatisation is best suited for your sample (alkylation, silylation or acylation)? Test kit for determination of the optimum derivatisation.
Screw Neck Vials N9
Screw neck vials for storage of liquid samples.Wide openingShort threadFlat bottom
LLG-Storage Boxes, PP
* With alphanumeric coding on the edge and the bottom of each cavity. Suitable for storage in the fridge. Temperature stable between -80 °C and 100 °C.
Snap ring vials N11
Snap ring / crimp neck vials N11 may be sealed either with a snap ring closure N11 or a crimp closure N11.
VarioPrep guard column holders and replacement parts
VarioPrep columnscolumn system for preparative HPLC manufactured from stainless steel with two adjustable end fittings, allows compensation of a dead volume, which could result at the column inlet after some time of operation, without need for opening the column, packed with NUCLEODUR® and NUCLEOSIL® spherical silica.O-rings available on request.
Accessories and replacement parts for EC columns
Analytical column system manufactured from stainless steel M 8 outer threads on both ends combination of sealing element and very fine-meshed stainless steel screen, PTFE sealing ring and fitting adaptor column heads SW 12 with inner threads M8 x 0,75 and UNF 10-32, as built -in guard columns use ChromCart® guard column cartridges with 8mm length with the guard column adaptor EC, packed with NUCLEODUR® spherical silica.
Glass Wool, quartz wool, glass fiber wadding
Glass wool, quartz wool and glass fiber wadding are, e.g., used for GC liners, packed GC columns etc.
NUCLEOGEL® SUGAR 810 Ca
Sulphonated polystyrene/divinylbenzene resins in diffent ionic forms and RP chromatographySeparation mechanism includes ion exclusion, ion exchange, size exclusion, ligand exchange as well as NP and RP chromatographyValco type columns for separation of mono-, di- and oliogosaccharides - USP L19 eluent in column water.
Guard columns for NUCLEOGEL® SUGAR 810 Ca columns
Sulphonated polystyrene/divinylbenzene resins in diffent ionic forms and RP chromatographySeparation mechanism includes ion exclusion, ion exchange, size exclusion, ligand exchange as well as NP and RP chromatographyThis guard columns require the CC column holder 30 mm.
NUCLEOGEL® SUGAR 810 H
Sulphonated polystyrene/divinylbenzene resins in diffent ionic forms and RP chromatographySeparation mechanism includes ion exclusion, ion exchange, size exclusion, ligand exchange as well as NP and RP chromatographyValco type columns for separation of sugars, sugar alcohols and organic acids - USP L17 eluent in column 0.01 N H2SO4
Guard columns for NUCLEOGEL® SUGAR 810 H columns
Sulphonated polystyrene/divinylbenzene resins in diffent ionic forms and RP chromatographySeparation mechanism includes ion exclusion, ion exchange, size exclusion, ligand exchange as well as NP and RP chromatographyThis guard columns require the CC column holder 30 mm.
EC analytical columns NUCLEOSIL® 100-5 C18 PAH, 5 µm
Special octadecyl phase for PAH analysis.Base material NUCLEOSIL® silica, particle size 5 µm, pore size 100 Å; polymeric coating - USP L1; eluent in column acetonitrile/water 70:30; allows efficient gradient separation of the 16 PAH in accordance with EPA.Detection of the separated PAH by UV (250 to 280 nm), with diode array or with fluorescence detection at different wavelegths for excitation and emission (acenaphthylene cannot be analysed with fluorescence detection).Guard columns for EC columns NUCLEOSIL® 100-5 C18 PAH, 5 µm available on request.
EC analytical columns NUCLEOCEL DELTA S, 5 µm
NUCLEOCEL DELTA enantiomer separation based on cellulose derivativesBase material silica,chiral selector Cellulosetris-(3,5-dimethylphenylcarbamate) - USP L40Similar phases: Chiralcel® OD, Kromasil®, CelluCoat™, Eurocel® 01S-Type for high resolution, allows use of shorter columns (150 mm) for faster separations, pressure stability up to ~ 150 bar (2000 psi).Recommended applications: pharmaceutically active compounds, chiral pollutants (e. g. herbicides, PCB), chiral compounds in food (dyes, preservatives), chiral catalysts and bioorganic compounds.NUCLEOCEL DELTA for normal phase applications:Eluent in column n-heptane - propanol-2 (90:10, v/v), particle size 5 µm.
EC analytical columns NUCLEOCEL DELTA-RP S, 5 µm
NUCLEOCEL DELTA enantiomer separation based on cellulose derivativesBase material silica,chiral selector Cellulosetris-(3,5-dimethylphenylcarbamate) - USP L40Similar phases: Chiralcel® OD, Kromasil®, CelluCoat™, Eurocel® 01S-Type for high resolution, allows use of shorter columns (150 mm) for faster separations, pressure stability up to ~ 150 bar (2000 psi).Recommended applications: pharmaceutically active compounds, chiral pollutants (e. g. herbicides, PCB), chiral compounds in food (dyes, preservatives), chiral catalysts and bioorganic compounds.NUCLEOCEL DELTA-RP for reversed phase applications:Eluent in column acetonitrile - water (40:60, v/v), particle size 5 µm. Designed for use either in polar organic mode or with eluents containing high concentrations of chaotropic salts such as perchlorate.
GC Capillary columns LIPODEX® E
LIPODEX® cyclodextrin phase for enantiomer separationBase material: cyclic oligosaccharide consisting of eight glucose units bonded through α-1,4-linkages (y-cylodextrin) regioselective alkylation and acylation of the hydroxyl groups leads to a lipophilic phase, which is well suited for GC enantiomer analyses; important advantage: many compounds can be analysed without derivatisation (however, for certain substances enantioselectivity can be favourably influenced by formation of derivatives).Octakis-(2,6-di-O-pentyl-3-O-butyryl)-γ-cyclodextrin. Recommended for α-amino acids, α- and β-hydroxycarboxylic acid esters, alcohols (TFA), diols (TFA), ketones, pheromones (cyclic acetals), amines, alkyl halides, lactones.Max. temperature for isothermal operation: 200 °CMax. temperature for short isotherms in a temperature programme: 220 °C
GC Capillary columns HYDRODEX β-6TBDM
HYDRODEX cyclodextrin phases for enantiomer separationHeptakis-(2,3-di-O-methyl-6-O-t-butyldimethyl-silyl)-β-cyclodextrin. Phase diluted with optimised polysiloxane recommended for γ-lactones, cyclopentanones, terpenes, esters, tartrates.Max. temperature for isothermal operation: 230 °CMax. temperature for short isotherms in a temperature programme: 250 °C
GC Capillary columns HYDRODEX β-TBDAC
HYDRODEX cyclodextrin phases for enantiomer separationHeptakis-(2,3-di-O-acetyl-6-O-t-butyldimethyl-silyl)-β-cyclodextrin. Phase diluted with optimised polysiloxane recommended for alcohols, esters, ketones, aldehydes, δ-lactones.Max. temperature for isothermal operation: 220 °CMax. temperature for short isotherms in a temperature programme: 240 °C